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Contact

Rawen Smirani

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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

3D-bioprinting of pre-vascularised autologous gingival substitutes for oral soft tissue regeneration

Abstract

Reference

Project Leader

Soft tissue grafts are used to enhance tissues around teeth and implants for issues like mucogingival deformities, insufficient tissue thickness, lack of keratinised tissue, and aesthetics (1). Literature highlights the importance of peri-implant soft tissues for long-term health (2). The most effective treatment for tissue deficiencies is autogenous grafts, including subepithelial connective tissue from the palate, but donor site morbidity limits their use. Alternatives like xenogeneic collagen matrices reduce morbidity and produce satisfactory results, though they suffer from shrinkage(3). Tissue engineering (TE) offers multi-layered tissue constructs aiming to mimic native tissues, with vascularisation being crucial for oxygen and nutrient supply (4).  

Strategies like growth factor scaffolds, cocultures of target and endothelial cells, and microfabrication are used for in vitro angiogenesis (5). ‘Pre-vascularisation' involves creating pre-vessel networks in constructs for faster host connection, especially relevant for gingival TE (6). Cocultures of human gingival fibroblasts and endothelial cells can improve vascularisation and repair (7,8). Cell-laden hydrogels have been used to fabricate vascularised oral mucosa, but pre-vascularisation remains challenging (9). 3D-bioprinting offers precise, customizable tissue fabrication by layer-by-layer deposition of cells and biomaterials, enabling tailored grafts. This project combines three strategies: cocultures of fibroblasts and endothelial cells, extrusion-based bioprinting to create channels and distribute cells, and short pre-incubation to develop pre-vessels (10).

▷Daniel S Thoma, Alfonso Gil, Christoph H F Hämmerle, Ronald E Jung. Management and prevention of soft tissue complications in implant dentistry. PMC9306802

▷William V Giannobile, Ronald E Jung, Frank Schwarz 3; Groups of the 2nd Osteology Foundation Consensus Meeting. Evidence-based knowledge on the aesthetics and maintenance of peri-implant soft tissues: Osteology Foundation Consensus Report Part 1-Effects of soft tissue augmentation procedures on the maintenance of peri-implant soft tissue health. PMID: 29498127

▷Jan Cosyn, Célien Eeckhout, Thomas De Bruyckere, Aryan Eghbali, Stijn Vervaeke, Faris Younes, Véronique Christiaens. A multi-centre randomized controlled trial comparing connective tissue graft with collagen matrix to increase soft tissue thickness at the buccal aspect of single implants: 1-year results. PMID: 35781692

▷Jeroen Rouwkema, Barto Koopman, Clemens Blitterswijk, Wouter Dhert, Jos Malda. Supply of nutrients to cells in engineered tissues. PMID: 21415880

▷Esther C Novosel, Claudia Kleinhans, Petra J Kluger. Vascularization is the key challenge in tissue engineering. PMID: 21396416

▷Rawen Smirani, Murielle Rémy, Raphael Devillard, Adrien Naveau. Engineered Prevascularization for Oral Tissue Grafting: A Systematic Review. PMID: 32597330

▷Nasar Um Min Allah, Zurairah Berahim, Azlina Ahmad, Thirumulu Ponnuraj Kannan. Biological Interaction Between Human Gingival Fibroblasts and Vascular Endothelial Cells for Angiogenesis: A Co-culture Perspective. PMID: 30603504

▷Smirani R , Rémy M , Devillard R , Naveau A. Use of Human Gingival Fibroblasts for Pre-Vascularization Strategies in Oral Tissue Engineering. PMID: 35048331

▷Daniela S Masson-Meyers a, Luiz E Bertassoni b, Lobat Tayebi a, Oral mucosa equivalents, prevascularization approaches and potential applications, PMID: 35132918

▷R Smirani, C Médina, J Becker, C Déchelette, B Rousseau, J-C Fricain, A Naveau, In vivo vessel connection of pre-vascularised 3D-bioprinted gingival connective tissue substitutes. PMID: 39837090