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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

Laser-assisted bioprinting for in situ bone regeneration

Abstract

Reference

Project Leader

Large bone defects caused by trauma, infection, tumors, or congenital disorders remain a major challenge in regenerative medicine (1). More than two million bone grafts are performed annually worldwide (2), yet current strategies remain limited. Autologous grafts, although considered the gold standard for their osteoconductive, osteoinductive, and osteogenic properties, suffer from donor-site morbidity, limited availability, and postoperative complications (3). Allogeneic and xenogeneic substitutes present immunological risks, while synthetic biomaterials often lack the biological signals required for proper integration and early vascularization (4).




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▷Zhang, J. et al. Research Progress of Bone Grafting: A Comprehensive Review. Int J Nanomedicine 20, 4729–4757 (2025).

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▷Polo-Corrales, L., Latorre-Esteves, M. & Ramirez-Vick, J. E. Scaffold design for bone regeneration. J Nanosci Nanotechnol 14, 15–56 (2014).

▷Santos, M. I. & Reis, R. L. Vascularization in bone tissue engineering: physiology, current strategies, major hurdles and future challenges. Macromol Biosci 10, 12–27 (2010).

▷Carulli, C., Innocenti, M. & Brandi, M. L. Bone vascularization in normal and disease conditions. Front Endocrinol (Lausanne) 4, 106 (2013).

▷Schott, N. G., Friend, N. E. & Stegemann, J. P. Coupling Osteogenesis and Vasculogenesis in Engineered Orthopedic Tissues. Tissue Eng Part B Rev 27, 199–214 (2021).

▷Moroni, L. et al. Biofabrication: A Guide to Technology and Terminology. Trends Biotechnol 36, 384–402 (2018).

▷Mironov, V., Boland, T., Trusk, T., Forgacs, G. & Markwald, R. R. Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 21, 157–161 (2003).

▷Gruene, M. et al. Laser printing of stem cells for biofabrication of scaffold-free autologous grafts. Tissue Eng Part C Methods 17, 79–87 (2011).

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▷Keriquel, V. et al. In vivo bioprinting for computer- and robotic-assisted medical intervention: preliminary study in mice. Biofabrication 2, 014101 (2010).


Raphaël Devillard

Olivia Kérourédan

Early vascularization is one of the key determinants of bone graft success (5), as insufficient vascular ingrowth frequently leads to necrosis and regeneration failure (6). This has motivated the development of pre-vascularization strategies combining vasculogenic cells, such as HUVECs or endothelial progenitor cells, with mesenchymal stem cells to promote coordinated angiogenesis and osteogenesis (7). In parallel, advances in biofabrication have enabled the controlled assembly of cells and biomaterials into biomimetic 3D constructs (8). Among these technologies, bioprinting provides precise spatial control of living cells (9). Laser-Assisted Bioprinting (LAB), in particular, offers high resolution, contact-free deposition, and excellent cell viability (10), making it a promising tool for bone tissue engineering. The BioTis unit has developed a dedicated LAB platform allowing precise in vitro printing and demonstrating the feasibility of in situ LAB directly onto bone defects (11,12). However, early outcomes highlighted the need to improve vascularization of printed constructs. This project aims to optimize bone regeneration using LAB by: i/ organizing vasculogenic and osteogenic cells into early microvascular networks; ii/ developing mineral-enriched hydrogels tailored for LAB; iii/ implementing in vivo LAB protocols for direct, localized bioprinting within bone defects. This approach seeks to establish a personalized, vascularized, in situ LAB-based strategy for maxillofacial bone regeneration.

Mina Medojević

Collaborator