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Contact

Hugo De Oliveira

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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

PACINIMO: Novel bioprinting approaches for the creation of advanced pancreatic cancer initiation models (ANR)

Abstract

Reference

Charles Handschin

Project Leader

The project uses general bioprinting approaches to precisely organize primary pancreatic acini, stromal cells and tailored biomaterial matrices into controlled 3D architectures, enabling high-throughput generation of “tumoroid” models that mimic key microenvironmental features such as extracellular matrix composition and rigidity, cell–cell and cell–matrix interactions, and hypoxic conditions. By systematically modulating these parameters and combining the models with live imaging and molecular analyses, the work will clarify how the acinar niche drives acinar-to-ductal metaplasia and progression towards pancreatic ductal adenocarcinoma. Beyond advancing the fundamental understanding of pancreatic cancer initiation, the resulting platform is conceived as a versatile preclinical tool for testing therapeutic and diagnostic strategies and can be adapted to the study of other cancer types.

▷Handschin C, Shalhoub H, Mazet A, Guyon C, Dusserre N, Boutet-Robinet E, Oliveira H, Guillermet-Guibert J. Biotechnological advances in 3D modeling of cancer initiation: Examples from pancreatic cancer research and beyond. Biofabrication. 2025;17(2):022008.

▷Desigaux T, Comperat L, Dusserre N, Stachowicz M-L, Lea M, Dupuy J-W, Vial A, Molinari M, Fricain J-C, Paris F, et al. 3D bioprinted breast cancer model reveals stroma-mediated modulation of extracellular matrix and radiosensitivity. Bioact Mater. 2024;42:316–327.

▷Douillet C, Nicodeme M, Hermant L, Bergeron V, Guillemot F, Fricain J-C, Oliveira H, Garcia M. From local to global matrix organization by fibroblasts: A 4D laser-assisted bioprinting approach. Biofabrication. 2022;14(2):025006.

▷Hakobyan D, Medina C, Dusserre N, Stachowicz M-L, Handschin C, Fricain J-C, Guillermet-Guibert J, Oliveira H. Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study. Biofabrication. 2020;12(3):035001.

This project aims to develop advanced three-dimensional in vitro models to study the earliest steps of pancreatic cancer initiation using innovative bioprinting and biofabrication strategies. Pancreatic ductal adenocarcinoma is one of the deadliest cancers, and there is a critical need for physiologically relevant models that reproduce the complexity of the tumor initiation niche while reducing reliance on animal experimentation.


Shane Fennell

Aurelien Mazet

Marie-Laure Stachowicz

Collaborator