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Contact

Claudine Boiziau

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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

Autophagy and mineralization

Abstract

Reference

Camille Blanchard

Project Leader

Autophagy, a process involved in the elimination of dysfunctional or useless intracellular components (Levine and Klionsky, 2004), plays an important role in cell differentiation (Adelipour et al., 2022). The alteration of this process created in the osteoblasts of transgenic mice by deletion of a key autophagy gene (ATG5 ko) leads to a deficiency in skeletal mineralization (Nollet et al., 2014).

The role of autophagy in mineralization produced by stem cells from apical papilla (SCAPs) in culture was studied by inhibiting this process in two different ways: i) treatment with drugs that transiently block the initiation of autophagy or fusion with lysosomes, and ii) genetic deletion of autophagy protein expression (ATG5 ko or ATG7 ko).




The results led to the hypothesis that the two known pathways of autophagy, canonical autophagy, long described by Y. Ohsumi (Ohsumi, 1999) and the alternative pathway more recently discovered in ATG5/7 ko cells (Nishida et al., 2009), could be involved in the transport of mineralized nodules into the extracellular environment (Le Nihouannen et al., 2025).

The project aims to study the impact of autophagy on mineralization and to clarify the pathways of secretion of calcium phosphate nodules.

▷Adelipour, M., Saleth, L.R., Ghavami, S., Alagarsamy, K.N., Dhingra, S., Allameh, A., 2022. The role of autophagy in the metabolism and differentiation of stem cells. Biochim. Biophys. Acta BBA - Mol. Basis Dis. 1868, 166412. https://doi.org/10.1016/j.bbadis.2022.166412

▷Le Nihouannen, D., Boiziau, C., Rey, S., Agadzhanian, N., Dusserre, N., Cordelières, F., Priault, M., Boeuf, H., 2025. Inhibiting Autophagy by Chemicals During SCAPs Osteodifferentiation Elicits Disorganized Mineralization, While the Knock-Out of Atg5/7 Genes Leads to Cell Adaptation. Cells 14, 146. https://doi.org/10.3390/cells14020146

▷Levine, B., Klionsky, D.J., 2004. Development by Self-Digestion. Dev. Cell 6, 463–477. https://doi.org/10.1016/S1534-5807(04)00099-1

▷Nishida, Y., Arakawa, S., Fujitani, K., Yamaguchi, H., Mizuta, T., Kanaseki, T., Komatsu, M., Otsu, K., Tsujimoto, Y., Shimizu, S., 2009. Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature 461, 654–658. https://doi.org/10.1038/nature08455

▷Nollet, M., Santucci-Darmanin, S., Breuil, V., Al-Sahlanee, R., Cros, C., Topi, M., Momier, D., Samson, M., Pagnotta, S., Cailleteau, L., Battaglia, S., Farlay, D., Dacquin, R., Barois, N., Jurdic, P., Boivin, G., Heymann, D., Lafont, F., Lu, S.S., Dempster, D.W., Carle, G.F., Pierrefite-Carle, V., 2014. Autophagy in osteoblasts is involved in mineralization and bone homeostasis. Autophagy 10, 1965–1977. https://doi.org/10.4161/auto.36182

▷Ohsumi, Y., 1999. Molecular mechanism of autophagy in yeast, Saccharomyces cerevisiae. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 354, 1577–1581. https://doi.org/10.1098/rstb.1999.0501


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