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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

Laser-assisted bioprinting for in situ bone regeneration

Abstract

Reference

Project Leader

Large bone defects caused by trauma, infection, tumors, or congenital disorders remain a major challenge in regenerative medicine [1]. More than two million bone grafts are performed annually worldwide [2], yet current strategies remain limited. Autologous grafts, although considered the gold standard for their osteoconductive, osteoinductive, and osteogenic properties, suffer from donor-site morbidity, limited availability, and postoperative complications [3]. Allogeneic and xenogeneic substitutes present immunological risks, while synthetic biomaterials often lack the biological signals required for proper integration and early vascularization [4].




▷[1] García-Gareta, E., Coathup, M. J. & Blunn, G. W. Osteoinduction of bone grafting materials for bone repair and regeneration. Bone 81, 112–121 (2015).

▷[2] Zhang, J. et al. Research Progress of Bone Grafting: A Comprehensive Review. Int J Nanomedicine 20, 4729–4757 (2025).

▷[3] Salgado, A. J., Coutinho, O. P. & Reis, R. L. Bone tissue engineering: state of the art and future trends. Macromol Biosci 4, 743–765 (2004).

▷[4] Polo-Corrales, L., Latorre-Esteves, M. & Ramirez-Vick, J. E. Scaffold design for bone regeneration. J Nanosci Nanotechnol 14, 15–56 (2014).

▷[5] Santos, M. I. & Reis, R. L. Vascularization in bone tissue engineering: physiology, current strategies, major hurdles and future challenges. Macromol Biosci 10, 12–27 (2010).

▷[6] Carulli, C., Innocenti, M. & Brandi, M. L. Bone vascularization in normal and disease conditions. Front Endocrinol (Lausanne) 4, 106 (2013).

▷[7] Schott, N. G., Friend, N. E. & Stegemann, J. P. Coupling Osteogenesis and Vasculogenesis in Engineered Orthopedic Tissues. Tissue Eng Part B Rev 27, 199–214 (2021).

▷[8] Moroni, L. et al. Biofabrication: A Guide to Technology and Terminology. Trends Biotechnol 36, 384–402 (2018).

▷[9] Mironov, V., Boland, T., Trusk, T., Forgacs, G. & Markwald, R. R. Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 21, 157–161 (2003).

▷[10] Gruene, M. et al. Laser printing of stem cells for biofabrication of scaffold-free autologous grafts. Tissue Eng Part C Methods 17, 79–87 (2011).

▷[11] Guillemot, F. et al. High-throughput laser printing of cells and biomaterials for tissue engineering. Acta Biomater 6, 2494–2500 (2010).

▷[12] Keriquel, V. et al. In vivo bioprinting for computer- and robotic-assisted medical intervention: preliminary study in mice. Biofabrication 2, 014101 (2010).


Dr. Raphaël Devillard

Olivia Kérourédan

Early vascularization is one of the key determinants of bone graft success [5], as insufficient vascular ingrowth frequently leads to necrosis and regeneration failure [6]. This has motivated the development of pre-vascularization strategies combining vasculogenic cells, such as HUVECs or endothelial progenitor cells, with mesenchymal stem cells to promote coordinated angiogenesis and osteogenesis [7]. In parallel, advances in biofabrication have enabled the controlled assembly of cells and biomaterials into biomimetic 3D constructs [8]. Among these technologies, bioprinting provides precise spatial control of living cells [9]. Laser-Assisted Bioprinting (LAB), in particular, offers high resolution, contact-free deposition, and excellent cell viability [10], making it a promising tool for bone tissue engineering. The BioTis unit has developed a dedicated LAB platform allowing precise in vitro printing and demonstrating the feasibility of in situ LAB directly onto bone defects [11,12]. However, early outcomes highlighted the need to improve vascularization of printed constructs. This project aims to optimize bone regeneration using LAB by: i/ organizing vasculogenic and osteogenic cells into early microvascular networks; ii/ developing mineral-enriched hydrogels tailored for LAB; iii/ implementing in vivo LAB protocols for direct, localized bioprinting within bone defects. This approach seeks to establish a personalized, vascularized, in situ LAB-based strategy for maxillofacial bone regeneration.

Mina Medojević

Collaborator